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Updated: Mar 25, 2026
Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology
Published on: May 23, 2025
Investigation of Helicobacter pylori antigen in stool samples of patients with upper gastrointestinal complaints
Zeki Calik1, Murat Karamese1, Osman Acar1
1Department of Microbiology, Faculty of Medicine, Kafkas University, Kars, Turkey.
Insights
The H. pylori Stool Antigen Test accurately detects Helicobacter pylori infection, showing high sensitivity and specificity. This noninvasive method offers a reliable alternative to invasive tests, especially for children.
Area of Science:
- Gastroenterology
- Microbiology
- Diagnostic Medicine
Background:
- Helicobacter pylori infection is a persistent global health concern, often acquired in childhood.
- Diagnosis typically relies on invasive methods, posing challenges, particularly in pediatric populations.
- Accurate and accessible diagnostic tools are crucial for effective management.
Purpose of the Study:
- To evaluate the diagnostic accuracy of the noninvasive H. pylori Stool Antigen Test.
- To compare the stool antigen test with the invasive Rapid Urease Test (RUT), the current gold standard.
- To assess the utility of the stool antigen test as an alternative diagnostic method.
Main Methods:
- A comparative study involving 122 patients with upper gastrointestinal complaints.
- Collection of both stool and gastric biopsy samples for analysis.
- Application of the H. pylori Stool Antigen Test on stool samples and the Rapid Urease Test on biopsy samples.
Main Results:
- H. pylori infection was confirmed in 106 out of 122 patients (86.8%).
- The stool antigen test demonstrated a sensitivity of 92.45% and specificity of 81.25%.
- Positive and negative predictive values were 97.02% and 61.90%, respectively.
Conclusions:
- The H. pylori Stool Antigen Test is a rapid, noninvasive, and cost-effective diagnostic tool.
- The test exhibits high sensitivity and specificity, making it a valuable alternative to invasive procedures.
- This method is particularly suitable for H. pylori detection in children and patients who cannot undergo endoscopy.
Abstract:
Helicobacter pylori infection is usually acquired in early childhood and it can persist throughout life without antibiotic treatment. This study aimed to compare the accuracy of the noninvasive H. pylori Stool Antigen Test-applied on the stool samples with the invasive gold standart Rapid Urease Test-applied on the gastric biopy samples of patients with upper gastrointestinal complaints. After endoscopy, biopsy and stool specimens were taken in 122 patients. The infection was detected with rapid urease test which is accepted as gold standart test. Rapid, one-step H. pylori card test was applied to all patients stool specimens. In this study 106 of the 122 patients (86.8%) were positive for H. pylori infection, while 16 of the 122 patients (13.2%) were negative. H. pylori card test was negative in 13 of the 16 patients and was positive in 98 of the 106. The sensitivity, specifity, positive and negative predictive values were 92.45%, 81.25%, 97.02%, and 61.90%, respectively. H. pylori card test is rapid, easy, noninvasive and inexpensive methods for detection H. pylori infection. This test showed high sensitivity and specificity. Additionally, it may be a good alternative to invasive tests for the detection of H. pylori infections especially in children.
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