Related Experiment Video
Updated: Mar 25, 2026

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
Published on: November 30, 2021
Demographic differences between funerary caves and megalithic graves of northern Spanish Late Neolithic/Early
Teresa Fernández-Crespo1,2, Concepción de-la-Rúa1
1Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV/EHU), Bº Sarriena S/N, Leioa, 48940, Bizkaia, Spain.
Objectives:
The study focuses on the estimation of demographic parameters of Late Neolithic/Early Chalcolithic (mid 4th-early 3rd millenniums cal. BC) burial sites from the La Rioja region (Ebro valley, northern Spain) to identify demographic characteristics.
Materials And Methods:
The human remains come from three caves (Las Yurdinas II, Peña Larga, and La Peña de Marañón) and three megalithic graves (Alto de la Huesera, San Martín, and Peña Guerra II). The total skeletal sample consists of a minimum of 261 individuals, 149 being buried in caves and 112 in megalithic graves. Data based on age and sex estimation are analyzed using abridged life tables, mortality rates, and sex ratios.
Results:
A systematic bias against children under 5 years of age is detected both in caves (5 q0 = 187.92%) and megalithic graves (5 q0 = 71.43%) but also against some juveniles and adults compared with population models, though a statistically significant greater lack of infants is worth noting in the megaliths (t-test, P = 0.012). Moreover, a significant divergence in sex ratios (χ(2) , P = 0.002) is also identified between site types, clearly prioritizing women in caves (sex ratio = 0.45) and men in megalithic graves (sex ratio = 1.33).
Conclusions:
This evidence is interpreted as the result of different selective burial patterns. The mortuary variability could lie behind intragroup differential status relationships, though the hypothesis of two populations performing distinct funerary practices in a small region cannot be rejected at the present state of the research. Am J Phys Anthropol 160:284-297, 2016. © 2016 Wiley Periodicals, Inc.
Related Concept Videos
Diversity of Archaea II
Diversity of Archaea III
Diversity of Archaea IV
Diversity of Archaea I
The Fossil Record
Changes in the Appendicular Skeleton with Age
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...

