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Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
Published on: September 15, 2016
Water-soluble poly(2,7-dibenzosilole) as an ultra-bright fluorescent label for antibody-based flow cytometry
Xin Wang1, Yi-Zhen Hu1, Aimei Chen1
1Biosciences Division, Thermo Fisher Scientific Inc. 29851 Willow Creek Rd., Eugene, OR 97402, USA. Kyle.Gee@thermofisher.com.
Abstract:
A series of novel water-soluble PEGylated dibenzosilole-based conjugated polymers were prepared as ultra-bright fluorescent labels for biomolecules. Due to their superior solubility and brightness, antibody conjugates labeled with functionalized polymers showed significantly enhanced signal and sensitivity relative to traditional fluorophores in functional flow cytometry applications.
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