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Updated: Mar 25, 2026

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

Phage-displayed macrocyclic glycopeptide libraries.

Simon Ng¹, Ratmir Derda¹

¹Alberta Glycomics Centre and Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada. ratmir@ualberta.ca.

Organic & Biomolecular Chemistry

|February 19, 2016

Summary

This summary is machine-generated.

This study presents a novel method for creating macrocyclic glycopeptide libraries efficiently. The technique uses phage-displayed peptides and dichloro-oxime derivatives, enabling genetic selection for glycan-binding proteins.

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Area of Science:

Biochemistry
Molecular Biology
Synthetic Chemistry

Background:

Macrocyclic glycopeptides are important in biological processes.
Generating diverse libraries of these molecules is challenging.
Phage display is a powerful tool for molecular selection.

Purpose of the Study:

To develop an efficient one-step method for generating libraries of macrocyclic glycopeptides.
To demonstrate the compatibility of the method with phage display technology.
To enable genetic selection of macrocyclic glycopeptides for specific glycan-binding proteins.

Main Methods:

Reaction of phage-displayed peptide libraries with dichloro-oxime derivatives.
Assessment of phage replication and viability post-reaction.
Evaluation of reaction site-selectivity on phage particles.

Main Results:

An efficient one-step method for generating macrocyclic glycopeptide libraries was established.
The chemical reactions did not impede phage replication in bacteria.
The reactions were site-selective for phage-displayed peptides, leaving other phage proteins unmodified.

Conclusions:

The described technology provides an efficient route to macrocyclic glycopeptide libraries.
This method is compatible with phage display and bacterial replication.
The approach will be instrumental in the genetic selection of macrocyclic glycopeptides targeting glycan-binding proteins.