Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent Advances in Vision-Based Beef Cattle Body Measurement Technologies.

Animals : an open access journal from MDPI·2026
Same author

Diagnostic performance of machine learning models based on dual-phase 99mTc-MIBI SPECT/CT semiquantitative parameters for differentiating benign and malignant pulmonary nodules.

PloS one·2026
Same author

Polymer-Gated Bilayer Buccoadhesive Tablets for Biphasic Release of Indomethacin: Balancing Dissolution and Mucoadhesion.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Discovery and validation of a prognostic SPP1/PLAU signature in HPV-negative oropharyngeal squamous cell carcinoma.

BMC medical genomics·2026
Same author

Rosmarinic acid ameliorates radiation-induced intestinal injury through PI3K/AKT/NF-κB/MMP9 axis inhibition: From network pharmacology prediction to experimental validation.

Tissue & cell·2026
Same author

The mechanisms of myricetin and quercetin in regulating miRNA-140 and MMP/TIMP signaling pathway in osteoarthritis treatment.

Pakistan journal of pharmaceutical sciences·2026

Related Experiment Video

Updated: Mar 25, 2026

Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces
10:21

Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces

Published on: July 26, 2016

12.1K

Potential Modulation on Total Internal Reflection Ellipsometry.

Wei Liu1,2,3, Yu Niu1,2, A S Viana4

  • 1NML, Institute of Mechanics, Chinese Academy of Sciences , 15 Bei-si-huan West Road, Beijing, 100190, China.

Analytical Chemistry
|February 19, 2016
PubMed
Summary

Electrochemical-total internal reflection ellipsometry (EC-TIRE) monitors electrode redox reactions. Potential modulation influences the TIRE response, showing a direct proportionality for nonfaradaic processes.

More Related Videos

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures
08:01

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Published on: November 21, 2019

7.8K
Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM
09:14

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM

Published on: October 2, 2012

10.7K

Related Experiment Videos

Last Updated: Mar 25, 2026

Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces
10:21

Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces

Published on: July 26, 2016

12.1K
Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures
08:01

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Published on: November 21, 2019

7.8K
Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM
09:14

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM

Published on: October 2, 2012

10.7K

Area of Science:

  • Electrochemistry
  • Surface Science
  • Optical Techniques

Background:

  • Electrochemical-total internal reflection ellipsometry (EC-TIRE) offers high phase sensitivity for electrolyte/electrode interfaces.
  • Observing redox reactions at electrode surfaces is crucial in electrochemistry.

Purpose of the Study:

  • To investigate the impact of potential modulation on the EC-TIRE response.
  • To understand the relationship between potential changes and optical signals at the electrode surface.

Main Methods:

  • Theoretical analysis of potential modulation effects on TIRE.
  • Experimental validation using linear sweep voltammetry.
  • Open circuit potential measurements.

Main Results:

  • Potential modulation affects TIRE response through gold dielectric constant variations and electric double-layer changes.
  • For nonfaradaic processes, the EC-TIRE signal is proportional to potential modulation.
  • Experimental results confirm the theoretical analysis.

Conclusions:

  • EC-TIRE is a sensitive technique for studying electrode surface phenomena.
  • Potential modulation is a key factor influencing EC-TIRE signals.
  • The study validates the theoretical framework for EC-TIRE analysis.