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Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Potential Modulation on Total Internal Reflection Ellipsometry.

Wei Liu^1,2,3, Yu Niu^1,2, A S Viana⁴

¹NML, Institute of Mechanics, Chinese Academy of Sciences , 15 Bei-si-huan West Road, Beijing, 100190, China.

Analytical Chemistry

|February 19, 2016

Summary

This summary is machine-generated.

Electrochemical-total internal reflection ellipsometry (EC-TIRE) monitors electrode redox reactions. Potential modulation influences the TIRE response, showing a direct proportionality for nonfaradaic processes.

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Area of Science:

Electrochemistry
Surface Science
Optical Techniques

Background:

Electrochemical-total internal reflection ellipsometry (EC-TIRE) offers high phase sensitivity for electrolyte/electrode interfaces.
Observing redox reactions at electrode surfaces is crucial in electrochemistry.

Purpose of the Study:

To investigate the impact of potential modulation on the EC-TIRE response.
To understand the relationship between potential changes and optical signals at the electrode surface.

Main Methods:

Theoretical analysis of potential modulation effects on TIRE.
Experimental validation using linear sweep voltammetry.
Open circuit potential measurements.

Main Results:

Potential modulation affects TIRE response through gold dielectric constant variations and electric double-layer changes.
For nonfaradaic processes, the EC-TIRE signal is proportional to potential modulation.
Experimental results confirm the theoretical analysis.

Conclusions:

EC-TIRE is a sensitive technique for studying electrode surface phenomena.
Potential modulation is a key factor influencing EC-TIRE signals.
The study validates the theoretical framework for EC-TIRE analysis.