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Updated: Mar 25, 2026

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Published on: June 12, 2017
Dysfunctional autophagy is a driver of muscle stem cell functional decline with aging
Laura García-Prat1, Pura Muñoz-Cánoves1,2, Marta Martinez-Vicente3
1a Cell Biology Group , Department of Experimental and Health Sciences , Pompeu Fabra University (UPF), CIBER on Neurodegenerative Diseases (CIBERNED) , Barcelona , Spain.
Abstract:
Regeneration of skeletal muscle relies on its resident stem cells, also known as satellite cells, which are normally quiescent. With aging, satellite cell quiescence is lost concomitant with a muscle regenerative decline. Here we demonstrate that autophagy sustains quiescence over time and that its failure with age drives senescence, which accounts for stem cell loss of function. Pharmacological and genetic reestablishment of autophagy restores homeostasis and regenerative functions in geriatric satellite cells, which has relevance for the elderly population.
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