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Updated: Jan 14, 2026
08:13
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
Published on: September 26, 2025
462
Ingression Progression Complexes Control Extracellular Matrix Remodelling during Cytokinesis in Budding Yeast
Magdalena Foltman1,2, Iago Molist1,2, Irene Arcones3
1Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria, CSIC, Santander, Spain.
Plos Genetics
|February 19, 2016
Summary
Researchers discovered
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Eukaryotic cytokinesis requires coordinated actomyosin ring contraction, plasma membrane ingression, and extracellular matrix (ECM) remodeling.
- Glycosyltransferases synthesizing ECM polysaccharides are increasingly recognized as crucial for cell division.
- In budding yeast, chitin synthase (Chs2) produces the primary septum, an essential ECM component for cell division.
Purpose of the Study:
- To elucidate the molecular mechanisms coordinating cell division processes in budding yeast.
- To identify and characterize protein complexes involved in cytokinesis.
- To understand the regulation of chitin synthase (Chs2) activity during cell division.
Main Methods:
- Isolation and characterization of protein complexes at the cleavage site during late cell cycle.
- Biochemical assays to determine protein-protein interactions and enzyme activation mechanisms.
- Experimental system to investigate the role of specific protein domains in regulating chitin synthase activity.
Main Results:
- Identification of 'ingression progression complexes' (IPCs) containing actomyosin ring components (myosin, Iqg1, Hof1) and cytokinesis regulators (Inn1, Cyk3) associated with chitin synthase (Chs2).
- Elucidation of chitin synthase activation mechanism via direct interaction of Inn1's C2 domain and Cyk3's transglutaminase-like domain with Chs2's catalytic domain.
- Discovery of Inn1's C-terminus preventing premature Chs2 activation, with Cyk3 mediating the release of this inhibition during late mitosis.
Conclusions:
- Ingression progression complexes (IPCs) are central to coordinating cell division in budding yeast.
- A novel regulatory mechanism for chitin synthase (Chs2) activation involving Inn1 and Cyk3 has been identified.
- These findings provide a detailed model for the spatiotemporal regulation of cell division through ECM synthesis.
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