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Serum myeloid-related protein 8/14 in minimal change- and glomerulonephritis-related nephrotic syndrome
Shinichiro Ohara1, Yukihiko Kawasaki2, Ryo Maeda1
1Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima City, Fukushima, Japan.
Background:
Myeloid-related protein 8/14 (MRP8/14) forms stable heterodimers and is the major calcium-binding protein secreted by activated granulocytes and monocytes. We evaluated whether serum MRP8/14 level is a useful indicator for a differential diagnosis of glomerulonephritis (GN)- and minimal change disease (MC)- related nephrotic syndrome (NS).
Methods:
Serum MRP8/14 complex was evaluated in 37 NS patients with MC or GN. These patients were divided into two groups. Group 1 consisted of 13 NS patients with MC, and group 2 consisted of 24 NS patients with GN. Group 2 was further divided into four subgroups: IgA nephropathy (IgAN; n = 5), Henoch-Schönlein purpura nephritis (HSPN; n = 6), focal segmental glomerulosclerosis (FSGS; n = 12), and acute GN Poststreptococcal acute glomeruloNephritis (PSAGN; n = 1).
Results:
The clinical manifestations, laboratory findings, serum MRP8/14 level, and renal accumulation of MRP8 were investigated for each group. No significant inter-group differences were observed for serum total protein, serum albumin, or blood urea nitrogen and urinary protein excretions. Mean serum MRP8/14 in the IgAN, HSPN, FSGS, and PSAGN groups was higher than in group 1. Further, the mean glomerular and interstitial MRP8 staining scores in the IgAN, HSPN, and PSAGN groups were higher than in group 1.
Conclusions:
Serum MRP8/14 level may be a useful indicator for differential diagnosis between GN- and MC- related NS.
Insights
Serum myeloid-related protein 8/14 (MRP8/14) levels can help differentiate between glomerulonephritis (GN) and minimal change disease (MC) in nephrotic syndrome (NS) patients. Elevated MRP8/14 indicates GN.
Area of Science:
- Nephrology
- Immunology
- Biochemistry
Background:
- Myeloid-related protein 8/14 (MRP8/14) is a calcium-binding protein secreted by activated immune cells.
- Nephrotic syndrome (NS) presents diagnostic challenges, particularly differentiating between glomerulonephritis (GN) and minimal change disease (MC).
Purpose of the Study:
- To investigate the utility of serum MRP8/14 levels in distinguishing GN from MC in NS patients.
- To assess MRP8/14 deposition in renal tissues of NS patients.
Main Methods:
- Serum MRP8/14 levels were measured in 37 NS patients (13 MC, 24 GN).
- GN patients were subcategorized into IgA nephropathy (IgAN), Henoch-Schönlein purpura nephritis (HSPN), focal segmental glomerulosclerosis (FSGS), and post-streptococcal acute GN (PSAGN).
- Renal tissue MRP8 staining was evaluated.
Main Results:
- No significant differences in standard renal function markers or proteinuria were found between groups.
- Mean serum MRP8/14 levels were significantly higher in all GN subgroups compared to MC.
- Increased glomerular and interstitial MRP8 staining was observed in IgAN, HSPN, and PSAGN compared to MC.
Conclusions:
- Serum MRP8/14 levels show potential as a biomarker for differentiating GN from MC in NS.
- Elevated MRP8/14 suggests underlying glomerulonephritis in nephrotic syndrome.
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