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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Related Experiment Video

Updated: Mar 25, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Miniaturization Technologies for Efficient Single-Cell Library Preparation for Next-Generation Sequencing.

Sergio Mora-Castilla¹, Cuong To¹, Soheila Vaezeslami²

¹Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.

Journal of Laboratory Automation

|February 20, 2016

Summary

This summary is machine-generated.

Researchers developed a cost-effective method for single-cell transcriptome sequencing by reducing reaction volumes. This technique maintains data quality and reproducibility for high-throughput applications.

Keywords:

library miniaturization scale-down single cell

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Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Next-generation sequencing costs have decreased, making library preparation a significant expense.
High-throughput applications like single-cell RNA profiling are particularly affected by library preparation costs.

Purpose of the Study:

To develop a novel, cost-effective method for high-throughput single-cell transcriptome sequencing.
To scale down reaction volumes for library preparation without compromising data quality.

Main Methods:

Utilized in vitro differentiated human embryonic stem cells for pancreatic differentiation studies.
Employed Fluidigm C1 system for single-cell complementary DNA (cDNA) generation.
Used an enzyme-based tagmentation system with a nanoliter liquid handler to reduce reaction volumes to 2 µL, requiring only 20 pg of input cDNA.

Main Results:

Decreasing reaction volume did not negatively impact sequencing data quality or reproducibility.
Transcriptional data from scaled-down libraries successfully distinguished between single cells.
The developed process demonstrated efficiency and cost-effectiveness for high-throughput applications.

Conclusions:

A novel, efficient, and cost-effective process for high-throughput single-cell transcriptome sequencing has been established.
Reduced reaction volumes are viable for library preparation in single-cell RNA profiling.
This method enables high-throughput analysis while reducing overall costs.