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Quantitative analysis of factor P (Properdin) in monkey serum using immunoaffinity capturing in combination with
Xinliu Gao1, Hui Lin1, Carsten Krantz2
1Department of Drug Metabolism & Pharmacokinetics, Novartis Institutes for BioMedical Research, East Hanover, NJ 07936, USA.
Bioanalysis
|February 20, 2016
Summary
A new method accurately quantifies Factor P (Properdin) in monkey serum, crucial for understanding drug effects in preclinical studies. This validated assay supports pharmacokinetic/pharmacodynamic research.
Area of Science:
- Immunology
- Biochemistry
- Pharmacology
Background:
- Factor P (Properdin) is a key glycoprotein in innate immunity.
- Accurate quantification of Factor P is essential for drug development and understanding pharmacodynamics (PD).
Purpose of the Study:
- To develop and validate a novel method for quantifying Factor P in monkey serum.
- To support preclinical pharmacokinetic/pharmacodynamic (PK/PD) studies.
Main Methods:
- Immunoaffinity capturing liquid chromatography-tandem mass spectrometry (LC-MS/MS).
- Method validation in factor P-depleted monkey serum.
Main Results:
- First validated LC-MS/MS method for Factor P quantification in monkey serum.
- Dynamic range: 125 to 25,000 ng/ml.
- High precision (≤7.2% CV) and accuracy (±16.8% Bias).
- Stability and other evaluations met acceptance criteria.
Conclusions:
- A robust and validated LC-MS/MS method for Factor P quantification.
- The method is suitable for supporting preclinical PK/PD studies.
