Related Experiment Videos
Immunochemical detection of Z-DNA in rat pachytene spermatocytes
1Department of Biochemistry, Indian Institute of Science, Bangalore.
Experimental Cell Research
|December 1, 1989
Abstract:
Rat testicular nuclei have been probed for the presence of Z-DNA conformation by employing indirect immunofluorescence technique using anti-Z-DNA antibodies. Pachytene nuclei, in which meiotic recombination takes place, showed brighter fluorescence than the premeiotic and postmeiotic spermatogenic nuclei. Moreover, utilizing a novel chromatin immunoblotting technique, Z-DNA conformation was found to be enriched in the poly(ADP-ribosyl)ated chromatin domains of the pachytene nucleus.