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Absence of immunosuppression in DBA/2 mice vaccinated with Trypanosoma cruzi treated with actinomycin-D
M Queiroz da Cruz1, H M Bräscher, J R Vargens
1Instituto de Puericultura e Pediatria de UFR, Rio de Janeiro, Brazil.
Abstract:
The subcutaneous (s.c.) vaccination of DBA/2 mice with 4 weekly doses of 3 x 10(7) living metacyclic forms of T. cruzi, Y strain, obtained from culture in axenic medium and treated for 24 h with actinomycin-D (50 micrograms/10(7) parasites), a drug that promotes an irreversible blockade of the parasite replication, do not induce any detectable degree of humoral and cellular immunosuppression as assessed by a) the production of anti-SRBC antibodies, b) the permanence of delayed cutaneous reaction to T. cruzi antigen, to PPD and DNCB and c) the degree of blastogenic transformation of spleen lymphocytes in the presence of the specific antigen.