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Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research
Published on: June 28, 2024
Amperometric Quantification of S-Nitrosoglutathione Using Gold Nanoparticles: A Step toward Determination of
Victor Baldim1,2,3,4,5, Abdulghani Ismail2,3,4,5, Patricia Taladriz-Blanco1
1Institute of Chemistry, University of Campinas , UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
Abstract:
S-Nitrosothiols (RSNOs) are carriers of nitric oxide (NO) and have important biological activities. We propose here the use of gold nanoparticles (AuNPs) and NO-selective amperometric microsensor for the detection and quantification of S-nitrosoglutathione (GSNO) as a step toward the determination of plasma RSNOs. AuNPs were used to decompose RSNOs with the quantitative release of free NO which was selectively detected with a NO microsensor. The optimal [GSNO]/[AuNPs] ratio was determined, corresponding to an excess of AuNP surface relative to the molar GSNO amount. Moreover, the influence of free plasma thiols on this method was investigated and a protocol based on the blocking of free thiols with iodoacetic acid, forming the carboxymethyl derivative of the cysteine residues, is proposed.
