Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

4.6K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simultaneous Atomic Resolution Imaging and Electronic Characterization of Wet-Chemically Prepared Nanocrystals.

Nano letters·2026
Same author

Measuring Local Exothermic Effects During the Oxidative Coupling of Methane Using Operando Luminescence Thermometry.

Angewandte Chemie (International ed. in English)·2026
Same author

In-Situ Ligand-Induced Chirality Transfer in Emissive CdSe Nanoplatelets.

The journal of physical chemistry letters·2026
Same author

Dose-Dependent and Irreversible Photodarkening of InP/ZnSe/ZnS Quantum Dots.

ACS nano·2026
Same author

Hot Injection Synthesis of Ultrathin Bi<sub>2</sub>Se<sub>3</sub> Nanosheets With Controllable Dimensions.

Small methods·2026
Same author

Coherent Electron-Phonon Coupling in Two-Dimensional Bi<sub>2</sub>Se<sub>3</sub> Nanoplatelets Studied with Ultrafast Spectroscopy.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026

Related Experiment Video

Updated: Mar 25, 2026

Observation and Analysis of Blinking Surface-enhanced Raman Scattering
05:52

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

7.8K

Non-blinking single-photon emitters in silica.

Freddy T Rabouw1, Nicole M B Cogan2, Anne C Berends1

  • 1Debye Institute for Nanomaterials Science, Utrecht University, Princetonplein 5, 3584 CC Utrecht, The Netherlands.

Scientific Reports
|February 20, 2016
PubMed
Summary

Glass microscope cover slips contain intrinsic fluorescent defects. These defects emit single photons and can be mistaken for quantum dots in spectroscopy experiments.

More Related Videos

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
10:33

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Published on: March 8, 2017

8.8K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.6K

Related Experiment Videos

Last Updated: Mar 25, 2026

Observation and Analysis of Blinking Surface-enhanced Raman Scattering
05:52

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

7.8K
Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
10:33

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Published on: March 8, 2017

8.8K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.6K

Area of Science:

  • Materials Science
  • Spectroscopy
  • Optics

Background:

  • Single-emitter spectroscopy typically uses spin-coated emitters on silicate glass cover slips.
  • Intrinsic optical properties of glass substrates are often overlooked in sample preparation.

Purpose of the Study:

  • To investigate the intrinsic fluorescence of borosilicate glass and quartz cover slips.
  • To determine if these intrinsic emissions interfere with single-emitter spectroscopy.
  • To characterize the optical properties of these intrinsic defect centers.

Main Methods:

  • Excitation of borosilicate glass and quartz cover slips at 532 nm.
  • Microscopic imaging to observe fluorescence.
  • Emission spectrum analysis to characterize the photoluminescence.
  • Photoluminescence lifetime measurements.

Main Results:

  • Both borosilicate glass and quartz exhibit intrinsic defect color centers that fluoresce at 532 nm.
  • The defect emission is spectrally indistinguishable from spin-coated emitters in microscope images.
  • The emission spectrum shows peaks between 2.05 and 2.20 eV, linked to silica vibrations.
  • These defects are non-blinking single-photon emitters with nanosecond lifetimes.

Conclusions:

  • Intrinsic defects in glass cover slips can be misinterpreted as single nanocrystal quantum dots.
  • Careful characterization is needed to distinguish intrinsic substrate fluorescence from actual emitters.
  • Understanding these defects is crucial for accurate single-emitter spectroscopy.