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Updated: Mar 25, 2026
Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Published on: January 5, 2015
Fluorescence optical imaging in anticancer drug delivery
Tomáš Etrych1, Henrike Lucas2, Olga Janoušková1
1Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic.
Abstract:
In the past several decades, nanosized drug delivery systems with various targeting functions and controlled drug release capabilities inside targeted tissues or cells have been intensively studied. Understanding their pharmacokinetic properties is crucial for the successful transition of this research into clinical practice. Among others, fluorescence imaging has become one of the most commonly used imaging tools in pre-clinical research. The development of increasing numbers of suitable fluorescent dyes excitable in the visible to near-infrared wavelengths of the spectrum has significantly expanded the applicability of fluorescence imaging. This paper focuses on the potential applications and limitations of non-invasive imaging techniques in the field of drug delivery, especially in anticancer therapy. Fluorescent imaging at both the cellular and systemic levels is discussed in detail. Additionally, we explore the possibility for simultaneous treatment and imaging using theranostics and combinations of different imaging techniques, e.g., fluorescence imaging with computed tomography.
Insights
Non-invasive imaging, particularly fluorescence imaging, is vital for understanding nanosized drug delivery systems in cancer therapy. This review explores its applications, limitations, and potential for theranostics.
Area of Science:
- Nanomedicine and Drug Delivery
- Biomedical Imaging
- Cancer Therapeutics
Background:
- Nanosized drug delivery systems are extensively researched for targeted delivery and controlled release.
- Understanding pharmacokinetics is essential for clinical translation of these systems.
- Fluorescence imaging is a prevalent tool in preclinical research for tracking nanomedicines.
Purpose of the Study:
- To review the applications and limitations of non-invasive imaging techniques in drug delivery, focusing on anticancer therapy.
- To detail the use of fluorescence imaging at cellular and systemic levels.
- To explore theranostics and multimodal imaging combinations for simultaneous treatment and imaging.
Main Methods:
- Review of current literature on non-invasive imaging techniques for nanomedicine.
- Detailed discussion of fluorescence imaging principles and applications in drug delivery.
- Exploration of theranostic concepts and combined imaging modalities (e.g., fluorescence and CT).
Main Results:
- Fluorescence imaging offers versatile applications for tracking nanocarriers in vitro and in vivo.
- Advancements in fluorescent dyes enhance imaging capabilities across visible to near-infrared spectra.
- Theranostics and combined imaging show promise for integrated diagnosis and therapy.
Conclusions:
- Non-invasive imaging, especially fluorescence imaging, is crucial for advancing nanomedicine in cancer treatment.
- Addressing limitations and exploring multimodal approaches will accelerate clinical applications.
- The integration of imaging and therapy via theranostics represents a significant future direction.
