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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
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Transcriptome analysis demonstrate widespread differential expression of long noncoding RNAs involve in Larimichthys
Lihua Jiang1, Wei Liu1, Aiyi Zhu1
1National Engineering Research Center of Maricultural Facilities of China, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, China.
Fish & Shellfish Immunology
|February 20, 2016
Summary
This study identifies long noncoding RNAs (lncRNAs) in the large yellow croaker fish. Researchers cataloged thousands of novel lncRNAs, with many specifically found in the spleen, suggesting roles in immune response.
Area of Science:
- Marine Biology
- Genomics
- Molecular Biology
Background:
- Long noncoding RNAs (lncRNAs) are crucial regulatory molecules in eukaryotes.
- Limited information exists on lncRNA expression and function in the large yellow croaker (Larimichthys crocea).
- Previous studies focused on protein-coding genes in this species.
Purpose of the Study:
- To create a comprehensive catalog of lncRNAs in the large yellow croaker.
- To investigate the tissue-specific expression patterns of lncRNAs.
- To identify potential immune-related lncRNAs in the spleen.
Main Methods:
- Integration of multiple RNA-sequencing datasets from various tissues (egg, muscle, liver, spleen).
- Bioinformatic analysis to reconstruct and identify high-confidence lncRNA transcripts.
- Comparative analysis of lncRNA expression profiles across different tissues.
Main Results:
- A total of 48,953 high-confidence lncRNA transcripts were identified across 38,017 loci.
- Thousands of novel lncRNA loci were discovered, expanding the known transcriptome.
- Most identified lncRNAs exhibited tissue-specific expression patterns.
- 210 lncRNAs were specifically enriched in the spleen, indicating potential immune functions.
Conclusions:
- This study provides the first systematic identification of lncRNAs in the large yellow croaker.
- The identified lncRNAs offer valuable resources for future genomic and functional studies in this species.
- Spleen-specific lncRNAs may play significant roles in the fish's immune response.
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