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Simultaneous quantitative analysis of nine vitamin D compounds in human blood using LC-MS/MS
Nur Sofiah Abu Kassim1, Fabio P Gomes1, Paul Nicholas Shaw1
1School of Pharmacy, The University of Queensland, QLD 4072, Australia.
Aim:
It has been suggested that each member of the family of vitamin D compounds may have different function(s). Therefore, selective quantification of each compound is important in clinical research.
Materials & Methods:
Development and validation attempts of a simultaneous determination method of 12 vitamin D compounds in human blood using precolumn derivatization followed by LC-MS/MS is described. Internal standard calibration with 12 stable isotope labeled analogs was used to correct for matrix effects in MS detector.
Results & Conclusion:
Nine vitamin D compounds were quantifiable in blood samples with detection limits within femtomole levels. Serum (compared with plasma) was found to be a more suitable sample type, and protein precipitation (compared with saponification) a more effective extraction method for vitamin D assay.
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