Related Experiment Video

Updated: Mar 25, 2026

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

12.0K

MicroRNA-26a induces osteosarcoma cell growth and metastasis via the Wnt/β-catenin pathway

Feng Qu1, Chun-Bao Li2, Bang-Tuo Yuan2

  • 1Department of Orthopedics, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China; Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.

Oncology Letters
|February 20, 2016
PubMed

Insights

MicroRNAs (miRNAs) regulate gene expression. This study found miR-26a is upregulated in osteosarcoma (OS) and promotes OS cell proliferation, migration, and invasion by targeting GSK-3β.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression.
  • Aberrant miRNA expression is common in human osteosarcoma (OS).

Purpose of the Study:

  • To investigate the role of miR-26a in osteosarcoma progression and metastasis.
  • To elucidate the molecular mechanism underlying miR-26a's function in OS.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR).
  • Luciferase activity assay, cell viability, migration, and invasion assays.
  • Western blot analysis and cell transfection.

Main Results:

  • miR-26a was significantly upregulated in OS tissues and cell lines.
  • Overexpression of miR-26a enhanced proliferation, migration, and invasion of OS Saos-2 cells.
  • miR-26a directly targets glycogen synthase kinase-3β (GSK-3β) by binding to its 3'-UTR, inhibiting GSK-3β expression.

Conclusions:

  • miR-26a plays a significant role in osteosarcoma proliferation.
  • miR-26a promotes OS progression by directly regulating the Wnt/β-catenin signaling pathway via GSK-3β inhibition.
Keywords:
cell proliferationglycogen synthase kinase-3βmicroRNA-26amicroRNAsosteosarcoma

More Related Videos

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

4.4K
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.9K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.9K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.6K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
9.2K