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Selective cell adhesion on femtosecond laser-microstructured polydimethylsiloxane.

A M Alshehri1, S Hadjiantoniou, R J Hickey

  • 1Department of Physics, University of Ottawa, 150 Louis Pasteur, Ottawa, Ontario K1N 6N5, Canada. Department of Physics, King Khalid University(KKU), PO Box 9004, Abha, Saudi Arabia.

Biomedical Materials (Bristol, England)
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Summary

Femtosecond laser treatment of polydimethylsiloxane (PDMS) controls cell growth by changing surface properties. Low energy laser pulses promote cell adhesion, while high energy pulses create superhydrophobic surfaces that inhibit it.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Surface Science

Background:

  • Controlling cell adhesion and growth on material surfaces is crucial for various biomedical applications.
  • Polydimethylsiloxane (PDMS) is a widely used polymer in microfluidics and tissue engineering.
  • Surface modification techniques can alter material properties to influence biological interactions.

Purpose of the Study:

  • To investigate the effect of femtosecond laser irradiation on PDMS surface properties.
  • To determine how laser-induced surface changes influence selective cell growth.
  • To explore the potential of laser patterning for creating cell-selective substrates.

Main Methods:

  • Femtosecond laser irradiation of PDMS at varying pulse energies.
  • Characterization of surface wettability using water contact angle measurements.
  • Analysis of surface chemical composition using X-ray photoelectron spectroscopy (XPS).
  • Assessment of cell adhesion (C2C12 cells) and protein attachment on laser-treated surfaces.

Main Results:

  • Low pulse energy irradiation resulted in decreased hydrophobicity and increased oxygen content.
  • Laser-treated regions with low pulse energy showed preferential attachment of C2C12 cells and proteins.
  • High pulse energy irradiation induced superhydrophobicity and nanoscale debris, inhibiting cell adhesion in treated areas.

Conclusions:

  • Femtosecond laser irradiation can precisely control PDMS surface properties, including wettability and chemistry.
  • Selective cell adhesion can be achieved by tuning laser parameters, enabling patterned cell growth.
  • This technique offers a promising approach for fabricating cell-instructive materials for biological applications.