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Related Experiment Videos

Intermediate filament collapse is an ATP-dependent and actin-dependent process.

P J Hollenbeck1, A D Bershadsky, O Y Pletjushkina

  • 1Medical Research Council Cell Biophysics Unit, London, England.

Journal of Cell Science
|April 1, 1989
PubMed
Summary

Intermediate filament collapse in mouse fibroblasts requires ATP for both stages. However, only the second stage, cable coiling, can be reversed without microtubules and actin interactions.

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Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Intermediate Filaments

Background:

  • Intermediate filaments, such as vimentin, are crucial cytoskeletal components.
  • Their dynamic rearrangements are essential for cellular processes.
  • Understanding vimentin filament behavior under stress is key to cell mechanics.

Purpose of the Study:

  • To investigate the mechanisms of intermediate filament rearrangements.
  • To elucidate the role of ATP and actin in vimentin filament collapse.
  • To differentiate the requirements for vimentin cable formation and perinuclear coiling.

Main Methods:

  • Experimentally induced collapse of vimentin intermediate filaments in mouse fibroblasts.
  • Depolymerization of microtubules using colchicine or vinblastine.

Related Experiment Videos

  • Inhibition of oxidative phosphorylation and glucose deprivation to lower intracellular ATP.
  • Treatment with cytochalasin D to disrupt actin filaments.

    • Main Results:

    • Intermediate filament collapse occurs in two stages: cable formation and perinuclear coiling.
    • Both stages of collapse are ATP-dependent.
    • Cable coiling, but not cable formation, can be reversed without microtubules by reducing ATP.
    • Cable formation is independent of actin, while coiling is inhibited by actin disruption.

    Conclusions:

    • Vimentin cable formation likely involves a phosphorylation event.
    • Perinuclear coiling of vimentin cables depends on interactions with the actin cortex.
    • Distinct molecular mechanisms govern the two stages of intermediate filament collapse.