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In vivo deep tissue imaging using wavefront shaping optical coherence tomography.

Hyeonseung Yu1, Peter Lee2, KyeoReh Lee1

  • 1Korea Advanced Institute of Science and Technology, Department of Physics, Daejeon 34141, Republic of KoreabKI for optical Science and Technology, Daejeon 34141, Republic of Korea.

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Wavefront shaping (WS) enhances optical coherence tomography (OCT) imaging penetration in live tissues. This technique optimizes light delivery, improving deep tissue visualization for biomedical applications.

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Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Biophotonics

Background:

  • Light scattering in biological tissues limits imaging depth in techniques like optical coherence tomography (OCT).
  • Overcoming scattering is crucial for deeper visualization of subsurface structures in vivo.
  • Wavefront shaping (WS) offers a potential solution to control light propagation through scattering media.

Purpose of the Study:

  • To investigate the efficacy of wavefront shaping (WS) for enhancing the penetration depth of spectral-domain optical coherence tomography (SD-OCT) in biological tissues.
  • To demonstrate in vivo OCT imaging in a live mouse model with improved deep tissue visualization using WS.
  • To optimize light delivery into deep tissue via complex WS for enhanced OCT performance.

Main Methods:

  • Implementation of a digital micromirror device (DMD) for complex wavefront shaping within a spectral-domain OCT system.
  • Ex vivo imaging of chicken breast and mouse ear tissues to assess signal strength and penetration depth improvements.
  • In vivo OCT imaging of a live mouse tail to evaluate deep tissue imaging capabilities.

Main Results:

  • Wavefront shaping significantly enhanced image signal strength and penetration depth in ex vivo tissue samples.
  • Successful in vivo OCT imaging of a live mouse tail was achieved, revealing multilayered tissue structures.
  • Optimized light energy delivery into deep tissue was confirmed through WS in the SD-OCT system.

Conclusions:

  • Wavefront shaping is an effective method for overcoming light scattering limitations in OCT.
  • This WS-enhanced OCT technique enables deeper and clearer imaging of biological tissues in vivo.
  • The study demonstrates the potential of WS-OCT for advanced biomedical imaging applications.