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Polyamines regulate cell growth and cellular methylglyoxal in high-glucose medium independently of intracellular
Min-Kyu Kwak1, Mun-Hyoung Lee1, Seong-Jun Park1
1Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
Abstract:
Polyamines can presumably inhibit protein glycation, when associated with the methylglyoxal inevitably produced during glycolysis. Herein, we hypothesized a nonenzymatic interaction between putrescine and methylglyoxal in putrescine-deficient or -overexpressing Dictyostelium cells in high-glucose medium, which can control methylglyoxal production. Putrescine was essentially required for growth rescue accompanying methylglyoxal detoxification when cells underwent growth defect and cell cycle G1-arrest when supplemented with high glucose. Furthermore, methylglyoxal regulation by putrescine seemed to be a parallel pathway independent of the changes in cellular glutathione content in high-glucose medium. Consequently, we suggest that Dictyostelium cells need polyamines for normal growth and cellular methylglyoxal regulation.
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