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Updated: Mar 25, 2026

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
Published on: April 2, 2020
Mammalian interspecies substitution of immune modulatory alleles by genome editing
Simon G Lillico1, Chris Proudfoot1, Tim J King1
1The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Abstract:
We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated with resilience to African Swine Fever. The ability to efficiently achieve interspecies allele introgression in one generation opens unprecedented opportunities for agriculture and basic research.
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