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Related Experiment Videos

Unusual enhancer function in yeast rRNA transcription.

S P Johnson1, J R Warner

  • 1Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.

Molecular and Cellular Biology
|November 1, 1989
PubMed
Summary

In yeast, a single enhancer significantly boosted transcription of tandem ribosomal RNA (rRNA) genes, regardless of its position. Multiple enhancers showed additive effects, suggesting a model for enhancer function in these gene arrays.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Eukaryotic Gene Regulation

Background:

  • Ribosomal RNA (rRNA) genes in eukaryotes are typically arranged in tandem arrays.
  • Evidence suggests that the transcription of individual rRNA genes within these arrays is interdependent.
  • Enhancers play a crucial role in regulating gene transcription, but their function in tandem gene arrays is not fully understood.

Purpose of the Study:

  • To investigate the relationship between the tandem arrangement of rRNA genes and their transcription.
  • To determine how enhancers influence the transcription of multiple, adjacent rRNA genes.
  • To elucidate the mechanism of enhancer function in tandemly repeated rRNA gene systems.

Main Methods:

  • Construction of a minirepeat containing two identifiable test rRNA genes.

Related Experiment Videos

  • Integration of the minirepeat into the URA3 locus in Saccharomyces cerevisiae.
  • Analysis of gene transcription by RNA polymerase I in the presence of one or two enhancers at various positions.

    • Main Results:

    • A single enhancer stimulated the transcription of both tandem rRNA genes 10- to 30-fold.
    • Enhancer effectiveness was maintained whether located upstream or downstream of the gene pair.
    • The presence of two enhancers resulted in approximately additive increases in transcription levels.

    Conclusions:

    • Enhancers can effectively coordinate the transcription of multiple rRNA genes in a tandem array.
    • The position of the enhancer relative to the tandem genes does not significantly impair its function.
    • These findings support a model where enhancers play a key role in regulating the expression of tandemly repeated genes.