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A fluorescent aptasensor for sensitive analysis oxytetracycline based on silver nanoclusters.

Morteza Hosseini1, Fatemeh Mehrabi2, Mohammad Reza Ganjali3,4

  • 1Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran. smhosseini@khayam.ut.ac.ir.

Luminescence : the Journal of Biological and Chemical Luminescence
|February 23, 2016
PubMed
Summary

A novel fluorescent aptasensor detects oxytetracycline (OTC) using DNA aptamer-templated silver nanoclusters. This sensitive and selective method offers rapid determination of OTC in honey and water samples.

Keywords:
aptamerfluorescencenanobiosensoroxytetracyclinequenchingsilver nanoclusters

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Area of Science:

  • Analytical Chemistry
  • Nanotechnology
  • Biotechnology

Background:

  • Oxytetracycline (OTC) is a widely used antibiotic with potential residues in food and water.
  • Accurate and rapid detection methods for OTC are crucial for food safety and environmental monitoring.
  • Existing detection methods may lack sensitivity, selectivity, or require complex procedures.

Purpose of the Study:

  • To develop a sensitive and selective fluorescent aptasensor for OTC detection.
  • To utilize DNA aptamer-templated silver nanoclusters (AgNCs) for fluorescence quenching-based detection.
  • To validate the aptasensor's performance in real-world samples like honey and water.

Main Methods:

  • Synthesis of DNA aptamer-templated silver nanoclusters (AgNCs) using a cytosine-rich DNA sequence.
  • Design of an OTC-specific aptamer fragment for selective OTC binding.
  • Fluorescence quenching assay based on the interaction between OTC, the aptamer, and AgNCs.

Main Results:

  • The aptasensor demonstrated a linear response to OTC concentration from 0.5 nM to 100 nM.
  • A low detection limit of 0.1 nM for OTC was achieved.
  • The developed nanobiosensor exhibited high sensitivity and selectivity for OTC detection.

Conclusions:

  • The fluorescent aptasensor based on DNA aptamer-templated AgNCs is a viable tool for OTC detection.
  • The method offers a sensitive, selective, and simple approach for rapid OTC determination.
  • This technology provides a promising alternative for monitoring OTC contamination in honey and water samples.