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Related Experiment Video

Updated: Mar 25, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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A multisample dissolution dynamic nuclear polarization system for serial injections in small animals.

Marcin Krajewski1,2, Patrick Wespi1, Julia Busch1

  • 1Institute for Biomedical Engineering, University and ETH Zurich, Switzerland.

Magnetic Resonance in Medicine
|February 23, 2016
PubMed
Summary

This study introduces a new four-sample dissolution dynamic nuclear polarization (DNP) setup for small animal research. The system enables rapid, serial injections of hyperpolarized [1-13C] pyruvate for in vivo metabolic studies.

Keywords:
dissolution DNPdynamic nuclear polarizationmetabolic imagingmulti-sample DNPsmall animal

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Area of Science:

  • Magnetic Resonance Imaging
  • Biomedical Engineering
  • Nuclear Physics

Background:

  • Dynamic Nuclear Polarization (DNP) enhances nuclear spin polarization for improved MRI sensitivity.
  • In vivo DNP applications necessitate rapid, sequential administration of hyperpolarized agents.
  • Existing DNP setups may not efficiently support rapid serial injections required for dynamic studies.

Purpose of the Study:

  • To design and evaluate a custom-built, multisample dissolution DNP setup for small animal research.
  • To enable rapid, successive injections of hyperpolarized substrates for in vivo studies.
  • To assess the performance and reproducibility of a novel four-sample DNP system.

Main Methods:

  • A custom-built DNP insert was developed, incorporating a 3.35 T magnet, cryostat, and 94 GHz microwave source.
  • The insert accommodates four sample sticks, each with a dissolver and a mechanism for sample cup retrieval.
  • Polarization levels were measured for [1-13C] pyruvate, and the system's in vivo applicability was tested in rats.

Main Results:

  • The setup achieved an average liquid-state polarization of 18.7% ± 2.3% for [1-13C] pyruvate 30 seconds post-dissolution.
  • A 75-90 minute window was available for dissolving three additional samples after the first.
  • Serial injections of [1-13C] pyruvate in rats demonstrated good reproducibility for cardiac metabolism studies.

Conclusions:

  • The developed four-sample DNP insert facilitates reproducible hyperpolarization of [1-13C] pyruvate.
  • The system supports rapid repeat injections, crucial for dynamic in vivo small animal research.
  • This technology advances the potential for DNP applications in preclinical metabolic imaging.