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Initiation of transcription on nucleosomal templates
1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Abstract:
We describe a simple method that uses curved DNAs to move nucleosomes relative to a sequence of interest. With this method, small changes in the association of a T7 RNA polymerase promoter with a nucleosome are shown to lead to significant changes in transcription efficiency.