Related Experiment Video
Updated: Mar 25, 2026
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Published on: July 17, 2018
Concentrations of adipokines in children before and after hematopoietic stem cell transplantation
Szymon Skoczen1, Przemyslaw J Tomasik2, Kamil Fijorek3
1a Department of Clinical Immunology, Chair of Clinical Immunology and Transplantation , Institute of Pediatrics, Jagiellonian University Medical College , Krakow , Poland.
Insights
Adipokines like leptin and adiponectin may indicate disease severity and inflammation in pediatric hematopoietic stem cell transplantation (HSCT). These adipokines might also predict metabolic complications following HSCT.
Area of Science:
- Endocrinology
- Hematology
- Metabolic Research
Background:
- Adipokines regulate crucial physiological processes including metabolism, inflammation, and cell growth.
- Pediatric hematopoietic stem cell transplantation (HSCT) can lead to significant metabolic complications.
- Understanding adipokine profiles in HSCT is essential for managing patient outcomes.
Purpose of the Study:
- To measure plasma concentrations of adiponectin, apelin, leptin, and resistin, and soluble leptin receptor in pediatric HSCT patients.
- To evaluate gene expression of these peptides using microarray analysis.
- To correlate adipokine levels with metabolic parameters and inflammation markers pre- and post-HSCT.
Main Methods:
- Plasma adipokine levels (adiponectin, apelin, leptin, resistin, soluble leptin receptor) measured before and after oral glucose tolerance test.
- Gene expression analysis via microarray.
- Measurement of fasting glucose, insulin, lipids, and hsCRP in pediatric HSCT patients (n=38) and healthy controls (n=26).
- Longitudinal assessment at pre-HSCT and 6 months post-HSCT.
Main Results:
- Pediatric HSCT patients exhibited an atherogenic lipid profile and low-grade systemic inflammation.
- Leptin, adiponectin, and resistin levels were associated with disease burden and inflammation.
- Observed changes in adipokine concentrations and gene expression warrant further investigation.
Conclusions:
- Adipokines, including leptin, adiponectin, and resistin, may serve as valuable biomarkers for disease burden and inflammation in pediatric HSCT.
- Adipokine dysregulation could play a role in the metabolic complications following HSCT.
- Further research is needed to determine if adipokine changes influence engraftment and cell line reconstitution.
Abstract:
Adipokines have multiple effects, including regulation of glucose metabolism, cell proliferation, inflammation, and angiogenesis. The aim of the study was to determine plasma concentrations of adiponectin, apelin, leptin, and resistin as well as soluble leptin receptor in pediatric hematopoietic stem cell transplantation (HSCT). The expression of genes encoding the studied peptides was measured using microarray technique. Plasma concentrations of tested peptides were measured before and after oral glucose tolerance test in children treated with HSCT (n = 38) and in healthy controls (n = 26). The peptides were measured before HSCT (pre-HSCT group; n = 38) and after a median of 6 months after HSCT (post-HSCT group; n = 27 of 38 children treated with HSCT). In addition, measurements of fasting plasma glucose, insulin, lipids, and high-sensitivity C-reactive protein (hsCRP) were performed. In both HSCT groups, atherogenic lipid profile, low-grade systemic inflammation was observed. Leptin, adiponectin, and resistin also appear to be good markers of disease burden and low-grade systemic inflammation. Adipokines may be good markers of disease burden and may influence metabolic complications of HSCT. Future studies on larger groups of patients will explain if changes of the concentrations of leptin, adiponectin, and apelin observed in our study and confirmed by expression levels influence engraftment and reconstitution of cell lines.
Related Concept Videos
01:01Regulation of Hematopoietic Stem Cells
01:28Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...
01:21Hematopoiesis