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Effect of Resorbable Collagen Plug on Bone Regeneration in Rat Critical-Size Defect Model
Weiqing Liu1, Ning Kang, Yuliang Dong
1*Graduate Student, State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China. †Lecturer, Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China. ‡Assistant Professor, Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. §Professor, State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
Objective:
The purpose of this investigation was to examine the effect of resorbable collagen plug (RCP) on bone regeneration in rat calvarial critical-size defects.
Methods:
About 5-mm-diameter calvarial defects were created in forty 12-week-old male Sprague-Dawley rats and implanted with or without RCP. Animals were killed at 1, 2, 4, and 8 weeks postoperatively. After being killed, specimens were collected and subjected to micro-computed tomography (μCT) and histological analysis.
Results:
The μCT showed a significant increase of newly formed bone volume/tissue volume in RCP-implanted defect compared with controls at all designated time points. After 8 weeks, the defects implanted with RCP displayed almost complete closure. Hematoxylin and eosin staining of the decalcified sections confirmed these observations and evidenced active bone regeneration in the RCP group. In addition, Masson's trichrome staining demonstrated that RCP implantation accelerated the process of collagen maturation.
Conclusions:
The RCP enhances bone regeneration in rat critical-size cranial defects, which suggest it might be a desired material for bone defect repair.
