Related Experiment Video
Updated: Mar 25, 2026
Retinal Vascular Reactivity as Assessed by Optical Coherence Tomography Angiography
Published on: March 26, 2020
Comparison of retinal vessel measurements using adaptive optics scanning laser ophthalmoscopy and optical coherence
Shigeta Arichika1, Akihito Uji2, Sotaro Ooto1
1Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan.
Purpose:
We compared adaptive optics scanning laser ophthalmoscopy (AOSLO) and optical coherence tomography (OCT) vessel caliber measurements.
Methods:
AOSLO videos were acquired from 28 volunteers with healthy eyes. Artery measurements were made 0.5-1 disc diameters away from the optic disc margin. Individual segmented retinal arterial caliber was measured in synchronization with cardiac pulsation and averaged to obtain final horizontal retinal arterial caliber (ACH) and horizontal retinal arterial lumen (ALH). All OCT images were obtained with the Spectralis OCT, a spectral-domain OCT system. Vertical retinal arterial caliber (ACV) and vertical retinal arterial lumen (ALV) were measured on the same artery measured with AOSLO. Measurements made with the two imaging systems were compared.
Results:
Average ACH, measured with AOSLO, was 123.4 ± 11.2 and average ALH was 101.8 ± 10.2 µm. Average ACV, measured with OCT, was 125.5 ± 11.4 and average ALV was 99.1 ± 10.6 µm. Both arterial caliber (r = 0.767, p < 0.0001) and arterial lumen (r = 0.81, p < 0.0001) measurements were significantly correlated between imaging modalities. Additionally, ACH and ACV were not significantly different (p = 0.16). However, ALH measurements were significantly higher than ALV measurements (p = 0.03).
Conclusions:
Vessel measurements made with AOSLO and OCT were well correlated. Moreover, plasma is visible and distinguishable from the retinal vessel wall in AOSLO images but not in OCT images. Therefore, AOSLO may measure vessel width more precisely than OCT.
