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Updated: Aug 19, 2026

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
Published on: September 25, 2013
Genetic determinants of hepatocarcinogenesis in the B6C3F1 mouse
N R Drinkwater1, M H Hanigan, C J Kemp
1McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Abstract:
The B6C3F1 mouse is highly susceptible to the induction of liver tumors because of the contribution of a specific gene, an allele of the Hcs (Hepatocarcinogen sensitivity) locus, inherited from its C3H inbred parent. This gene affects the rate of growth of preneoplastic hepatic lesions and results in the more rapid appearance of hepatic neoplasms in mice carrying the C3H allele in comparison to mice homozygous for the resistant C57BL/6 allele. The Hcs locus also acts synergistically with at least one class of chemical tumor promoters, the halogenated aromatic hydrocarbons. Because of this genetic promotion of hepatocarcinogenesis, B6C3F1 mice are more sensitive to liver tumor induction by both genotoxic and non-genotoxic carcinogens.
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