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Updated: Mar 25, 2026

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
Published on: August 31, 2016
Characterization data of gilthead sea bream (Sparus aurata) IGF-I receptors (IGF-IRa/Rb)
Emilio J Vélez1, Sheida Azizi2, Cristina Salmerón1
1Departament de Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal 643, 08028 Barcelona, Spain.
Abstract:
In this data article we describe the coding sequence of two IGF-IR paralogues (IGF-IRa and IGF-IRb) obtained from gilthead sea bream embryos. The putative protein architecture (domains and other important motifs) was determined and, amino acid sequences alignment and phylogenetic analysis of both receptors together with IGF-IR orthologues from different vertebrates was performed. Additionally, a semi-quantitative conventional PCR was done to analyze the mRNA expression of both receptors in different tissues of gilthead sea bream. These data will assist in further physiological studies in this species. In this sense, the expression of both receptors during ontogeny in muscle as well as the differential effects of IGF-I and IGF-II on their regulation during in vitro myogenesis has been recently studied (doi: 10.1016/j.ygcen.2015.11.011; [1]).
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