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Expression analysis and association of RBBP6 with apoptosis in colon cancers
Zodwa Dlamini1, Charleen Rupnarain2, Sarala Naicker3
1Research, Innovation and Engagements Portfolio, Mangosuthu University of Technology, Durban, 4031, South Africa. dlaminiz@mut.ac.za.
Journal of Molecular Histology
|February 25, 2016
Summary
The RBBP6 gene, a novel regulator of apoptosis, is upregulated in colon cancer. The RBBP6-3 isoform may promote cancer cell death, offering potential therapeutic targets.
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Background:
- Apoptosis regulation is crucial for preventing tumor progression.
- The RBBP6 gene interacts with p53 and Rb, and is linked to ubiquitin ligases.
- RBBP6 is a potential cancer biomarker and therapeutic target.
Purpose of the Study:
- To investigate RBBP6 gene transcription and expression patterns of its three isoforms in colon cancer.
- To evaluate the role of RBBP6 isoforms in apoptosis within colon cancer tissues.
Main Methods:
- Colorimetric and fluorescent in situ hybridization to localize RBBP6 mRNA transcripts in normal and cancerous colon tissue.
- Immunohistochemistry to localize RBBP6 protein isoforms in normal and cancerous colon tissues.
Main Results:
- All RBBP6 transcripts were upregulated in cancerous colon tissues.
- RBBP6-1 and RBBP6-3 (DWNN) protein expression increased in cancerous structures.
- Increased RBBP6-3 expression correlated with higher apoptosis levels and decreased Bcl-2 expression.
Conclusions:
- The RBBP6-3 isoform may play a role in promoting apoptosis in colon cancer cells.
- RBBP6 gene expression patterns offer insights into colon cancer development and potential therapeutic strategies.
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