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A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

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Segmented flow sampling with push-pull theta pipettes.

Anumita Saha-Shah¹, Curtis M Green¹, David H Abraham¹

¹Department of Chemistry, Indiana University, 800 E. Kirkwood Avenue, Bloomington, IN 47405, USA. lanbaker@indiana.edu.

|February 25, 2016

Summary

This summary is machine-generated.

We developed a mobile theta pipette microfluidic device for precise sample collection and direct electrospray mass spectrometry analysis. This technology enables high-resolution chemical monitoring in biological samples, revealing cellular heterogeneity.

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Area of Science:

Analytical Chemistry
Microfluidics
Biotechnology

Background:

Microfluidic devices offer precise control over small sample volumes.
Direct analysis of biological samples can be challenging due to heterogeneity and small sample sizes.

Purpose of the Study:

To develop a mobile and easily fabricated microfluidic device for segmented flow sampling.
To utilize theta pipettes as electrospray emitters for direct mass spectrometry analysis of sub-nanoliter sample segments.
To investigate intercellular heterogeneity in Allium cepa cells.

Main Methods:

Fabrication of a mobile theta pipette microfluidic device.
Use of theta pipettes for segmented flow sampling and electrospray ionization.
Direct electrospray mass spectrometry (ESI-MS) of collected sample segments.
Analysis of cytoplasm from multiple Allium cepa cells.

Main Results:

The theta pipette microfluidic device enabled manipulation and sampling of aqueous segments from 200 pL to tens of nanoliters.
Direct electrospray from theta pipette tips delivered analytes to the mass spectrometer without intermediate transfer steps.
High spatial and temporal resolution sampling was achieved, suitable for chemical monitoring.
Intercellular heterogeneity in Allium cepa was successfully studied via high-throughput analysis of collected cytoplasm.

Conclusions:

Theta pipette microfluidic devices are effective for high-resolution sampling and direct analysis.
This approach facilitates rapid, high-throughput investigation of sample heterogeneity in biological systems.
The technology is promising for chemical monitoring in biological samples.