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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Updated: Mar 25, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

Pyrosequencing on a glass surface.

Ana V Almeida¹, Andreas Manz, Pavel Neužil

¹KIST-Europe, Microfluidics Group, Campus E7.1, 66111 Saarbrücken, Germany. ana.almeida@kist-europe.de manz@kist-europe.de pavel.neuzil@gmail.com.

|February 25, 2016

Summary

This summary is machine-generated.

Open-surface microfluidics enables simple, low-cost DNA sequencing using pyrosequencing on a glass slide. This adaptable method integrates sample preparation and PCR for a complete "sample-to-answer" system.

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Last Updated: Mar 25, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping

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A Method of Targeted Cell Isolation via Glass Surface Functionalization

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

Area of Science:

Biotechnology
Analytical Chemistry
Microfluidics

Background:

Traditional DNA sequencing methods can be complex and expensive.
Microfluidic systems offer potential for miniaturization and cost reduction in molecular biology.

Purpose of the Study:

To demonstrate DNA sequencing by pyrosequencing using open-surface microfluidics.
To highlight the advantages of simplicity, disposability, and integration offered by this approach.

Main Methods:

Utilized a hydrophobically coated glass coverslip as the sequencing surface.
Employed superparamagnetic particles for DNA manipulation within micro-droplets.
Used mineral oil to cover droplets, preventing evaporation during pyrosequencing and washing steps.

Main Results:

Successfully performed DNA resequencing using 34-mer single-stranded DNA (ssDNA).
Achieved de novo sequencing with 51-mer and 81-mer ssDNA.
Demonstrated the potential for integrating sample preparation and PCR for a complete system.

Conclusions:

Open-surface microfluidics provides a simple, cost-effective platform for DNA pyrosequencing.
The method is highly adaptable and can be integrated into a fully automated sample-to-answer system.
This approach offers significant advantages in instrument size, simplicity, and disposability.