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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Molecular Characterization of Transgenic Events Using Next Generation Sequencing Approach.

Satish K Guttikonda1, Pradeep Marri1, Jafar Mammadov1

  • 1Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, Indiana, 46268, United States of America.

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Summary
This summary is machine-generated.

Next-generation sequencing (NGS) offers a faster, more cost-effective method for genetically modified (GM) crop molecular characterization. This study validates NGS approaches for regulatory safety assessments, improving upon traditional techniques.

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Biotechnology

Background:

  • Global population growth necessitates increased food production, driving demand for genetically modified (GM) crops.
  • Rigorous molecular characterization of GM crops is essential for regulatory safety assessments.
  • Traditional methods like Southern blot analysis are time- and resource-intensive.

Purpose of the Study:

  • To evaluate the efficacy of next-generation sequencing (NGS) for molecular characterization of GM crops.
  • To compare NGS-based methods with traditional Southern blot analysis for regulatory submissions.
  • To demonstrate the application of whole genome and target capture sequencing for transgenic event characterization.

Main Methods:

  • Application of whole genome sequencing (WGS) for molecular characterization.
  • Utilization of target capture sequencing (TCS) for molecular characterization.
  • Comparison of NGS results with traditional Southern blot analysis and Sanger sequencing.

Main Results:

  • NGS provides a highly sensitive, cost- and labor-effective alternative to traditional methods.
  • Successful characterization of single and stacked transgenic events using WGS and TCS.
  • NGS approaches yield comparable and reliable data for regulatory requirements.

Conclusions:

  • NGS technologies are a viable and advantageous alternative for molecular characterization of GM crops.
  • These methods streamline the safety assessment process for regulatory submissions.
  • NGS enhances the efficiency and accuracy of transgenic crop characterization.