Related Experiment Video
Updated: Mar 25, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Biodiversity and technological-functional potential of lactic acid bacteria isolated from spontaneously fermented
L Ruiz Rodríguez1, E Vera Pingitore2, G Rollan1
1Centro de Referencia para Lactobacilos (CERELA-CONICET), S. M. de Tucumán, Tucumán, Argentina.
Aims:
To analyse lactic acid bacteria (LAB) diversity and technological-functional and safety properties of strains present during spontaneous fermented quinoa sourdoughs.
Methods And Results:
Fermentation was performed by daily backslopping at 30°C for 10 days. Autochthonous LAB microbiota was monitored by a biphasic approach combining random amplified polymorphic DNA (RAPD)-PCR and rRNA gene sequencing with PCR-denaturing gradient gel electrophoresis (DGGE) analysis. Identification and intraspecies differentiation allowed to group isolates within nine LAB species belonging to four genera. A succession of LAB species occurred during 10-days backslopping; Lactobacillus plantarum and Lactobacillus brevis were detected as dominant species in the consortium. The characterization of 15 representative LAB strains was performed based on the acidifying capacity, starch and protein hydrolysis, γ-aminobutyric acid and exopolysaccharides production, antimicrobial activity and antibiotic resistance.
Conclusion:
Strains characterization led to the selection of Lact. plantarum CRL1905 and Leuconostoc mesenteroides CRL1907 as candidates to be assayed as functional starter culture for the gluten-free (GF) quinoa fermented products.
Significance And Impact Of The Study:
Results on native LAB microbiota present during quinoa sourdough fermentation will allow the selection of strains with appropriate technological properties to be used as a novel functional starter culture for GF-fermented products.
Related Concept Videos
Microbes in the Production of Fermented Foods
Microbes in Food Production
Production of Organic Acids
Microorganisms in Agriculture and Food industry
Bioreactor Controls-III
Microbes in Beverage Production

