[Development of a Monoclonal Antibody-Based Sensor for Environmental Pyrene and Benzo (a) pyrene Detection]

Polycyclic aromatic hydrocarbons (PAHs) are common environmental pollutants which are concerned because several of its members have been known to be carcinogenic and mutagenic. Pyrene, as a kind of PAHs, was covalently linked to carrier protein bovine serum albumin by active ester method and immunized to mice. A sensitive mAb 6A6 was developed by a three-step screening procedure. The IC50 of 6 A6 against pyrene and benzo(a) pyrene were 8.1 μg x L(-1) and 6.8 μg x L(-1), respectively. The cross-reactivity values (CR) with other PAHs were less than 5%. An immunosensor was developed based on 6A6 for pyrene and benzo(a) pyrene detection with a linear range of 0.2-10.0 μg x L(-1). The assessment of spiked water samples by the sensor demonstrated faster and more sensitive than ELISA method. We believe that this sensor can be used to rapidly assess pyrene and benzo(a) pyrene in environmental samples.