Related Experiment Video
Updated: Mar 25, 2026
10:22
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
Published on: June 29, 2021
4.1K
"Siglec"ting the allergic response for therapeutic targeting
1Department of Medicine, Division of Allergy-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA bruce.bochner@northwestern.edu.
Glycobiology
|February 26, 2016
Summary
Researchers explored glycobiology to understand immune cell differences, leading to the discovery of Siglec-8. This finding is crucial for developing new therapies for allergic and immunologic diseases.
Area of Science:
- Translational immunology
- Glycobiology
- Glycoimmunology
Background:
- Investigated mechanisms of human diseases involving eosinophils, basophils, mast cells, and neutrophils.
- Compared immune cell subsets to identify unique inflammatory pathways.
- Noticed functional differences related to selectins and their ligands.
Purpose of the Study:
- Delineate novel mechanisms of human disease.
- Identify therapeutic targets for allergic and immunologic diseases.
- Explore the role of glycobiology in immune cell function.
Main Methods:
- Utilized primary human cells and tissues for research.
- Compared eosinophils, basophils, mast cells, and neutrophils.
- Investigated selectin-ligand interactions and their functional consequences.
Main Results:
- Discovered Siglec-8, an I-type lectin expressed on human eosinophils, basophils, and mast cells.
- Identified Siglec-8 as a potential therapeutic target.
- Established fruitful collaborations with glycobiology experts.
Conclusions:
- Glycobiology significantly advanced understanding of immune cell function and disease mechanisms.
- Siglec-8 represents a promising target for therapeutic development in allergic and immunologic diseases.
- Further research into Siglec-8 and its ligands holds potential for novel treatment strategies.
Related Concept Videos
01:25Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
2.3K
Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
2.3K
01:27Allergic Drug Reactions
1.6K
Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing...
1.6K
01:10Antibody Structure
67.3K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
67.3K
02:06Allergic Reactions
33.4K
Overview
33.4K
01:30Allergic Reactions: Anaphylaxis
149
Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin,...
149
01:30Drug Toxicity: Allergic Reactions
129
Drug-related allergies are immune-mediated responses triggered by the administration of pharmacological agents. These hypersensitivity reactions are classified based on the immune mechanisms involved. The four primary types—Type I, II, III, and IV—are mediated by different immunological pathways and exhibit distinct clinical manifestations.Type I Hypersensitivity/ IgE-Mediated Reactions: Immunoglobulin E (IgE) immediately mediates Type I hypersensitivity reactions. Upon initial...
129