Related Experiment Video
Updated: Mar 25, 2026
Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons
Published on: September 14, 2016
Calcium imaging of sleep-wake related neuronal activity in the dorsal pons
Julia Cox1, Lucas Pinto1, Yang Dan1
1Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, 230 Li Ka Shing Center, Berkeley, California 94720, USA.
Abstract:
The dorsal pons has long been implicated in the generation of rapid eye movement (REM) sleep, but the underlying circuit mechanisms remain poorly understood. Using cell-type-specific microendoscopic Ca(2+) imaging in and near the laterodorsal tegmental nucleus, we found that many glutamatergic neurons are maximally active during REM sleep (REM-max), while the majority of GABAergic neurons are maximally active during wakefulness (wake-max). Furthermore, the activity of glutamatergic neurons exhibits a medio-lateral spatial gradient, with medially located neurons more selectively active during REM sleep.
