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Development of a rapid recombinase polymerase amplification assay for detection of Brucella in blood samples
Hang Ren1, Mingjuan Yang1, Guoxia Zhang2
1Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Molecular and Cellular Probes
|February 26, 2016
Abstract:
A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis.
