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Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Effects of lipopolysaccharide chemotype structure on binding and inactivation of hen egg lysozyme
1Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City.
Abstract:
The structural features of lipopolysaccharide [LPS] which influence the binding and inactivation of lysozyme have been examined. Binding of polysaccharide-containing LPS (S-LPS) and Ra-Rc-LPS preparations was independent of temperature between 37-50 degrees C; in contrast, binding of Rd-LPS, Re-LPS and lipid A was temperature-dependent. The binding of lysozyme to Rd-LPS and Re-LPS was increased by treatment with mild alkali, which has little detectable effect on binding of lysozyme to S-LPS and Ra-Rc-LPS preparations. Competitive binding experiments using dansylated lysozyme and/or dansylated polymyxin B indicated independent binding sites on the LPS for lysozyme and polymyxin B. These results indicate significant differences between most LPS preparations and lipid A and glycolipid LPS in their interaction with proteins of mammalian origin.
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