Specific 125I-endothelin-1 binding sites in the atrioventricular node of the porcine heart
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H Yamasaki1, M Niwa, K Yamashita
1Department of Pharmacology, Nagasaki University School of Medicine, Japan.
The quantitative receptor autoradiographic method we used revealed that specific 125I-endothelin-1 binding sites are highly concentrated in the atrioventricular node of the porcine heart. 125I-Endothelin-1 binding to the atrioventricular node, interatrial and interventricular septa was displaced by unlabeled endothelin-1 with Kd values of 53 pM, 2.03 nM and 3.48 nM, respectively. Knowledge of the existence of specific 125I-endothelin-1 binding sites in the porcine heart helps with understanding the physiology of endothelin, a possible endogenous Ca2+ channel agonist.
The quantitative receptor autoradiographic method we used revealed that specific 125I-endothelin-1 binding sites are highly concentrated in the atrioventricular node of the porcine heart. 125I-Endothelin-1 binding to the atrioventricular node, interatrial and interventricular septa was displaced by unlabeled endothelin-1 with Kd values of 53 pM, 2.03 nM and 3.48 nM, respectively. Knowledge of the existence of specific 125I-endothelin-1 binding sites in the porcine heart helps with understanding the physiology of endothelin, a possible endogenous Ca2+ channel agonist.