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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Mar 25, 2026

Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens

Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

Viral diagnosis in Indian livestock using customized microarray chips.

Brijesh S Yadav¹, Mayank Pokhriyal¹, Barkha Ratta¹

¹Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, India - 243122.

|February 26, 2016

Summary

This summary is machine-generated.

Customized microarray chips enable rapid viral diagnosis in Indian livestock, identifying Bovine herpes virus-1, Canine Adeno Virus-1, and Canine Parvo Virus-2. This technology offers a viable alternative for disease outbreak surveillance when traditional methods fall short.

Keywords:

DNA microarray RT-PCR livestock viral diagnosis

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Last Updated: Mar 25, 2026

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Area of Science:

Veterinary Virology
Molecular Diagnostics
Biotechnology

Background:

Viral diseases pose a significant threat to Indian livestock populations.
Accurate and rapid diagnostic tools are crucial for managing outbreaks and ensuring animal health.
Existing diagnostic methods may be insufficient during widespread viral disease events.

Purpose of the Study:

To evaluate the feasibility of using customized microarray chips for diagnosing viral infections in Indian livestock.
To identify specific viruses affecting livestock in India using this novel diagnostic approach.
To assess the potential of microarray technology in managing viral disease outbreaks.

Main Methods:

Design and application of customized microarray chips for viral detection.
Identification of specific viral pathogens including Bovine herpes virus-1 (BHV-1), Canine Adeno Virus-1 (CAV-1), and Canine Parvo Virus-2 (CPV-2).
Confirmation of microarray-identified probes using Reverse Transcription Polymerase Chain Reaction (RT-PCR).

Main Results:

Customized microarray chips successfully identified BHV-1, CAV-1, and CPV-2 in clinical samples from Indian livestock.
RT-PCR confirmed the accuracy of the microarray-based viral identification.
The study demonstrated the potential of microarray chips for diagnosing viral infections in diverse livestock populations.

Conclusions:

Customized microarray chips are a promising tool for the rapid and accurate diagnosis of viral diseases in Indian livestock.
This technology can be effectively applied during viral disease outbreaks, especially when conventional methods are not suitable.
Further cost-efficiency analysis is recommended for widespread implementation of customized microarray diagnostics.