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Updated: Mar 25, 2026
Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
Published on: September 27, 2024
Histologic study of bone-forming capacity on polydeoxyribonucleotide combined with demineralized dentin matrix
Seok-Kon Kim1, Chang-Kyu Huh2, Jae-Hoon Lee2
1Department of Pain and Anesthesiology, College of Medicine, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam Korea.
Background:
This study examined the osteoinductive activity of demineralized dentin matrix (DDM) from human and polydeoxyribonucleotide (PDRN) for nude mice.
Methods:
Twenty healthy nude mice, weighing about 15~20 g, were used for the study. DDM from human and PDRN were prepared and implanted subcutaneously into the dorsal portion of the nude mice. The nude mice were sacrificed at 1, 2, and 4 weeks after grafting and evaluated histologically by hematoxylin-eosin and Masson's trichrome staining. The specimens were also evaluated via a histomorphometric study.
Results:
The DDM and PDRN induced new bone, osteoblasts, and fibroblasts in soft tissues. The histological findings showed bone-forming cells like osteoblasts and fibroblasts at 1, 2, and 4 weeks. New bone formation was observed in the histomorphometric study. In particular, the ratio of new bone formation was the highest at 2 weeks compared with the first week and fourth week.
Conclusions:
In this study, we showed that the PDRN used in this experimental model was able to induce bone regeneration when combined to the DDM.
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