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Molecular Cloning and Expression Analysis of Pig Cd90
Kyungmin Park1, Jonggun Kim1, Chang-Yong Choi1
1a Department of Biotechnology , College of Life Sciences and Biotechnology , Korea University , Seoul , Republic of Korea.
Animal Biotechnology
|February 26, 2016
Summary
Researchers cloned pig CD90 (Thy-1) cDNA, revealing its sequence and expression in various tissues. This glycosylphosphatidylinositol-anchored glycoprotein plays multicellular roles and is recognized by anti-human CD90 antibodies.
Area of Science:
- Veterinary Immunology
- Molecular Biology
- Biochemistry
Background:
- CD90 (Thy-1) is a GPI-anchored glycoprotein involved in cell signaling, activation, migration, adhesion, and tumor suppression.
- Understanding CD90's role in pigs is crucial for veterinary research and disease management.
Purpose of the Study:
- To clone and sequence pig CD90 cDNA.
- To investigate the expression patterns of CD90 mRNA and protein in various pig tissues.
- To confirm the cross-reactivity of anti-human CD90 antibodies with pig CD90.
Main Methods:
- CDNA cloning and sequencing
- Reverse transcription polymerase chain reaction (RT-PCR) for mRNA detection
- Flow cytometry for cell surface protein analysis
- Immunohistochemistry for tissue expression profiling
Main Results:
- The complete cDNA sequence of pig CD90 was determined, encoding 161 amino acids with conserved structural features.
- Pig CD90 mRNA was detected in multiple tissues, indicating broad biological functions.
- Anti-human CD90 antibody successfully recognized pig CD90 on cell surfaces.
- Immunohistochemistry confirmed widespread CD90 protein distribution in pig tissues.
Conclusions:
- Pig CD90 shares conserved structural and expression characteristics with CD90 from other mammalian species.
- CD90 plays multicellular roles in pigs, with potential implications in health and disease.
- Further research is warranted to elucidate CD90's specific functions in pig infectious diseases.
