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[Phosphate starvation derepressed expression vector for engineering oleaginous yeast Rhodosporidium toruloides]
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|February 27, 2016
Summary
A novel phosphate starvation-inducible promoter (pPHO89) was developed for Rhodosporidium toruloides. This promoter enables efficient gene expression in oleaginous yeast for biofuel production.
Area of Science:
- Biotechnology
- Molecular Biology
- Synthetic Biology
Context:
- Oleaginous yeasts like Rhodosporidium toruloides are key for producing fatty acid-derived biofuels and biochemicals.
- Efficient genetic engineering tools are crucial for optimizing these yeasts for industrial applications.
Purpose:
- To construct a novel phosphate starvation-derepressed expression vector for Rhodosporidium toruloides.
- To characterize the pPHO89 promoter and tHSP terminator for gene expression regulation.
Summary:
- Bioinformatic analysis identified the inorganic phosphate symporter promoter (pPHO89) and heat shock protein terminator (tHSP).
- These elements were integrated into a new expression vector, pZPK-pPHO89-hyg-tHSP, using restriction-free cloning.
- Agrobacterium-mediated transformation (ATMT) successfully introduced the vector into R. toruloides, with hygromycin resistance confirming gene expression.
Impact:
- The pPHO89 promoter demonstrates effective regulation by phosphate levels, showing activation under phosphate-limited conditions.
- This study provides new inducible expression vectors for Rhodosporidium toruloides, facilitating metabolic engineering for biofuel and biochemical production.
- The developed vectors offer a cost-effective and efficient tool for yeast engineering.
