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[A case of acute mixed leukemia with expression of J5 and GPIIb/IIIa complex by ABC method]
Identification of the lineage and maturation stage of the leukemic cells is essential for the diagnosis, treatment and followup of acute leukemia. Staining of blood smear by avidin-biotin-peroxidase complex method, which requires no special equipments, enables to observe both morphology and surface antigens at the same time. Simultaneous expression of a B-lymphocyte antigen J5 and a platelet antigen GP IIb/IIIa complex on the same blasts was demonstrated by this technique in the present case.
Identification of the lineage and maturation stage of the leukemic cells is essential for the diagnosis, treatment and followup of acute leukemia. Staining of blood smear by avidin-biotin-peroxidase complex method, which requires no special equipments, enables to observe both morphology and surface antigens at the same time. Simultaneous expression of a B-lymphocyte antigen J5 and a platelet antigen GP IIb/IIIa complex on the same blasts was demonstrated by this technique in the present case.