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Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium - Short communication
Szabolcs Nagy1, Balázs Kakasi2, Miklós Bercsényi1
1Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia , Deák F. u. 16, H-8360 Keszthely , Hungary.
Abstract:
The aim of the present pilot study was to apply a flow cytometric assay, the so-called OxyDNA test, to determine the level of oxidative DNA damage in fish spermatozoa exposed to different concentrations (0.01-10,000 mg/L) of cadmium. Milt was collected from three randomly selected Prussian carp (Carassius auratus gibelio) males. Oxidative DNA damage was assessed with the OxyDNA kit and using flow cytometry. The ratio of OxyDNA-positive events increased significantly at higher cadmium concentrations. The results indicate that direct contact of fish spermatozoa with cadmium-polluted water initiates genotoxic damage.
